Microbiologia e Imunologia

Principais Atividades

Ensino de 1.º, 2.º e 3.º ciclos.
Investigação científica e desenvolvimento tecnológico.
Serviços de extensão universitária:
            serviços ao exterior
            atividades complementares no domínio da formação contínua e da promoção da inovação.


João Manuel Braz Gonçalves

Comissão de Departamento
A Comissão de Departamento é constituída pelos Doutores do DMI com vínculo à FFUL.



Instituto de Investigação do Medicamento (iMed.ULisboa)
Grupos de Investigação:
            Microbiologia Molecular e Biotecnologia
            Interações Hospedeiro Patogeno
            Evolução, Epidemiologia e Prevenção da Infeção por HIV




  • Amaral AJ, Andrade J, Foxall RB, Matoso P, Matos AM, Soares RS, et al. miRNA profiling of human naive CD4 T cells links miR-34c-5p to cell activation and HIV replication. EMBO Journal. 2017;36(3):346–60.
  • Anes E. Acting on Actin During Bacterial Infection. In: Jose C. Jimenez-Lopez, editor. Cytoskeleton - Structure, Dynamics, Function and Disease. London: InTech; 2017. p. 257–78.
  • Antunes F, Brito PM. Quantitative biology of hydrogen peroxide signaling. Redox Biology. 2017;13:1–7.
  • Araujo F, Goncalves J, Fonseca JE. Immunogenicity of biosimilars for the treatment of inflammatory rheumatic diseases: a review from confirmatory clinical trials. Annals of the Rheumatic Diseases. 2017 Jun;76(2):830.
  • Araujo FC, Fonseca JE, Goncalves J. The Explosion of Biosimilars in Immune Mediated Chronic Inflammatory Diseases. Current Pharmaceutical Design. 2017;23(44):6731–2.
  • Barber RM, Fullman N, Sorensen RJD, Bollyky T, McKee M, Nolte E, et al. Healthcare Access and Quality Index based on mortality from causes amenable to personal health care in 195 countries and territories, 1990-2015: a novel analysis from the Global Burden of Disease Study 2015. Lancet. 2017 Jul;390(10091):231–66.
  • Batuca J, Lamy M, Neves M, Batista F, Paiva-Lopes MJ, Valverde AH, et al. Anti-apolipoprotein A-I (APOA-I) antibodies have different target epitopes in different clinical conditions. Atherosclerosis. 2017;263:E216–7.
  • Bettencourt P, Carmo N, Pires D, Timóteo P, Anes E. Mycobacterial infection of macrophages: the effect of the multiplicity of infection. In: Antimicrobial Research: Novel bioknowledge and educational programs. Badajóz: Formatex Research Center;
  • Borrego P, Gonçalves MF, Gomes P, Araújo L, Moranguinho I, Brito Figueiredo I, et al. Assessment of the cavidi exavir load assay for monitoring plasma viral load in HIV-2-infected patients. Journal of Clinical Microbiology. 2017;55(8):2367–79.
  • Cantante C, Lourenço S, Morais M, Leandro J, Gano L, Silva N, et al. Albumin-binding domain from Streptococcus zooepidemicus protein Zag as a novel strategy to improve the half-life of therapeutic proteins. Journal of Biotechnology. 2017;253:23–33.
  • Cascao R, Carvalho T, Goncalves J, Moita LF, Fonseca JE. Efficacy and safety of oral administration of pure celastrol in aia rats. Annals of the Rheumatic Diseases. 2017 Jun;76(2):1080.
  • Cavaco M, Araujo F, Eurico Fonseca J, Goncalves J. TNF antagonist drug safety assessment by pharmacovigilance signaling and post-marketing adverse event reports. Annals of the Rheumatic Diseases. 2017 Jun;76(2):784–5.
  • Cavaco M, Goncalves J. Interactions Between Therapeutic Proteins and Small Molecules: The Shared Role of Perpetrators and Victims. Clinical Pharmacology and Therapeutics. 2017;102(4):649–61.
  • Cunha DV, Salazar SB, Lopes MM, Mira NP. Mechanistic insights underlying tolerance to acetic acid stress in vaginal Candida glabrata clinical isolates. Frontiers in Microbiology. 2017;8(FEB).
  • Fernandes S, São-José C. Probing the function of the two holin-like proteins of bacteriophage SPP1. Virology. 2017;500:184–9.
  • Figueira TN, Freire JM, Cunha-Santos C, Heras M, Goncalves J, Moscona A, et al. Quantitative analysis of molecular partition towards lipid membranes using surface plasmon resonance. Scientific Reports. 2017 Mar;7.
  • Fullman N, Barber RM, Abajobir AA, Abate KH, Abbafati C, Abbas KM, et al. Measuring progress and projecting attainment on the basis of past trends of the health-related Sustainable Development Goals in 188 countries: An analysis from the Global Burden of Disease Study 2016. The Lancet. 2017;390(10100):1423–59.
  • Gigante AM, Hampton CM, Dillard RS, Gil F, Catalão MJ, Moniz-Pereira J, et al. The Ms6 mycolyl-arabinogalactan esterase lysB is essential for an efficient mycobacteriophage-induced lysis. Viruses. 2017;9(11).
  • Goncalves J, Matos de Brito P, Batista A, Feio J, Machado F, Aperta J, et al. Position Paper from the Portuguese Association of Hospital Pharmacists for biosimilar therapeutic antibodies. Journal of Clinical Pharmacy and Therapeutics. 2017;42(2):239–43.
  • Gouveia Z, Carlos AR, Yuan X, Aires-da-Silva F, Stocker R, Maghzal GJ, et al. Characterization of plasma labile heme in hemolytic conditions. FEBS Journal. 2017;284(19):3278–301.
  • Gubareva LV, Besselaar TG, Daniels RS, Fry A, Gregory V, Huang W, et al. Global update on the susceptibility of human influenza viruses to neuraminidase inhibitors, 2015–2016. Antiviral Research. 2017;146:12–20.
  • Hay SI, Abajobir AA, Abate KH, Abbafati C, Abbas KM, Abd-Allah F, et al. Global, regional, and national disability-adjusted life-years (DALYs) for 333 diseases and injuries and healthy life expectancy (HALE) for 195 countries and territories, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017;390(10100):1260–344.
  • Kraemer MUG, Faria NR, Reiner Jr. RC, Golding N, Nikolay B, Stasse S, et al. Spread of yellow fever virus outbreak in Angola and the Democratic Republic of the Congo 2015-16: a modelling study. Lancet Infectious Diseases. 2017 Mar;17(3):330–8.
  • Machado D, Coelho TS, Perdigão J, Pereira C, Couto I, Portugal I, et al. Interplay between mutations and efflux in drug resistant clinical isolates of Mycobacterium tuberculosis. Frontiers in Microbiology. 2017;8(APR)
  • Maltez F, Martins T, Póvoas D, Cabo J, Peres H, Antunes F, et al. Multidrug-resistant tuberculosis by strains of beijing family, in patients from Lisbon, Portugal: Preliminary report | Tuberculose multirresistente por estirpes da família beijing, em doentes de Lisboa, Portugal: Estudo preliminar. Acta Medica Portuguesa. 2017;30(3):175–84
  • Martin F, Palladino C, Mateus R, Bolzan A, Gomes P, Brito J, et al. Early infant diagnosis of HIV-1 infection in Luanda, Angola, using a new DNA PCR assay and dried blood spots. PLoS ONE. 2017;12(7)
  • Naghavi M, Abajobir AA, Abbafati C, Abbas KM, Abd-Allah F, Abera SF, et al. Global, regional, and national age-sex specifc mortality for 264 causes of death, 1980-2016: A systematic analysis for the Global Burden of Disease Study 2016. The Lancet. 2017;390(10100):1151–210
  • Nakatani Y, Opel-Reading HK, Merker M, Machado D, Andres S, Kumar SS, et al. Role of alanine racemase mutations in Mycobacterium tuberculosis D-cycloserine resistance. Antimicrobial Agents and Chemotherapy. 2017;61(12)
  • Neves A, Marto J, Duarte A, Goncalves LM, Pinto P, Figueiredo AC, et al. Characterization of Portuguese Thymbra capitata, Thymus caespititius and Myrtus communis essential oils in topical formulations. Flavour and Fragrance Journal. 2017;32(5):392–402
  • Palladino C, Sánchez-Carrillo M, Mate-Cano I, Vázquez-Morón S, Jimenez-Sousa MÁ, Gutiérrez-Rivas M, et al. Low frequency of NS5A relevant resistance-associated substitutions to Elbasvir among hepatitis C virus genotype 1a in Spain: A cross-sectional study. Scientific Reports. 2017;7(1)
  • Penazzato M, Palladino C, Sugandhi N. Prioritizing the most needed formulations to accelerate paediatric antiretroviral therapy scale-up. Current Opinion in HIV and AIDS. 2017;12(4):369–76
  • Piedade D, Azevedo-Pereira JM. MicroRNAs as important players in host-adenovirus interactions. Frontiers in Microbiology. 2017;8(JUL)
  • Pires D, Bernard EM, Pombo JP, Carmo N, Fialho C, Gutierrez MG, et al. Mycobacterium tuberculosis modulates miR-106b-5p to control Cathepsin S expression resulting in higher pathogen survival and poor T-cell activation. Frontiers in Immunology. 2017;8(DEC)
  • Rezayan AH, Hariri S, Azerang P, Ghavami G, Portugal I, Sardari S. Synthesis of novel fluorene bisamide derivatives via ugi reaction and evaluation their biological activity against Mycobacterium species. Iranian Journal of Pharmaceutical Research. 2017;16(2):724–52
  • Rocha C, Duarte J, Borrego P, Calado R, Marcelino JM, Tendeiro R, et al. Potency of HIV-2-specific antibodies increase in direct association with loss of memory B cells. AIDS. 2017;31(17):2431–3
  • Rolim I, Duarte N, Barata G, Costa J, Gardete-Correia L, Boavida J, et al. Immunoglobulin M gene association with autoantibody reactivity and type 1 diabetes. Immunogenetics. 2017;69(7):429–37
  • Romero-Severson EO, Bulla I, Hengartner N, Bartolo I, Abecasis A, Azevedo-Pereira JM, et al. Donor-Recipient Identification in Para- and Poly-phyletic Trees Under Alternative HIV-1 Transmission Hypotheses Using Approximate Bayesian Computation. Genetics. 2017 Nov;207(3):1089–101
  • Santos FMF, Matos AI, Ventura AE, Gonçalves J, Veiros LF, Florindo HF, et al. Modular Assembly of Reversible Multivalent Cancer-Cell-Targeting Drug Conjugates. Angewandte Chemie - International Edition. 2017;56(32):9346–50
  • Subtil J, Rodrigues JC, Reis L, Freitas L, Filipe J, Santos A, et al. Adenoid bacterial colonization in a paediatric population. European Archives of Oto-Rhino-Laryngology. 2017;274(4):1933–8
  • São-José C, Santos MA, Schmitt MJ. Viruses of wine-associated yeasts and bacteria. In: König H., Unden G., Fröhlich J., editors. Biology of Microorganisms on Grapes, in Must and in Wine. Cham: Springer; 2017. 133-154 p.
  • Vos T, Abajobir AA, Abbafati C, Abbas KM, Abate KH, Abd-Allah F, et al. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: A systematic analysis for the Global Burden of Disease Study 2016. The Lancet. 2017;390(10100):1211–59
  • Wang H, Abajobir AA, Abate KH, Abbafati C, Abbas KM, Abd-Allah F, et al. Global, regional, and national under-5 mortality, adult mortality, age-specific mortality, and life expectancy, 1970-2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet. 2017;390(10100):1084–150
  • Xiong S, Borrego P, Ding X, Zhu Y, Martins A, Chong H, et al. A helical short-peptide fusion inhibitor with highly potent activity against human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus. Journal of Virology. 2017;91(1)


  • Araujo F, Goncalves J, Eurico Fonseca J. Cost savings with biosimilars in Rheumatology: a systematic literature Search Of Budget Impact Analysis. Annals of the Rheumatic Diseases. 2016 Jun;75(2):879–80.
  • Araújo F, Gonçalves J, Fonseca JE. Biosimilar DMARDs: What Does the Future Hold? Drugs. 2016;76(6):629–37.
  • Araújo FC, Gonçalves J, Fonseca JE. Pharmacoeconomics of Biosimilars: What Is There to Gain from Them? Current Rheumatology Reports. 2016;18(8).
  • Azevedo-Pereira JM, Santos-Costa Q. HIV interaction with human host: HIV-2 As a model of a less virulent infection. AIDS Reviews. 2016;18(1):44–53.
  • Berthenet E, Sheppard S, Vale FF. Recent “omics” advances in Helicobacter pylori. Helicobacter. 2016;21:14–8.
  • Bettencourt P, Pires D, Anes E. Immunomodulating microRNAs of mycobacterial infections. Tuberculosis. 2016 Mar;97:1–7.
  • Bártolo I, Calado R, Borrego P, Leitner T, Taveira N. Rare HIV-1 subtype J genomes and a new H/U/CRF02-AG recombinant genome suggests an ancient origin of HIV-1 in Angola. AIDS Research and Human Retroviruses. 2016;32(8):822–8.
  • Chen C-Y, Shingai M, Borrego P, Taveira N, Strebel K. Antagonism of BST-2/tetherin is a conserved function of primary HIV-2 Env glycoproteins. Retrovirology. 2016;13(1).
  • Chen C-Y, Shingai M, Welbourn S, Martin MA, Borrego P, Taveira N, et al. Antagonism of BST-2/tetherin is a conserved function of the Env glycoprotein of primary HIV-2 isolates. Journal of Virology. 2016;90(24):11062–74.
  • Cunha-Santos C, Figueira TN, Borrego P, Oliveira SS, Rocha C, Couto A, et al. Development of synthetic light-chain antibodies as novel and potent HIV fusion inhibitors. AIDS. 2016;30(11):1691–701.
  • Doering M, Borrego P, Buech J, Martins A, Friedrich G, Camacho RJ, et al. Geno2pheno (coreceptor-hiv2): a new diagnostic tool for the genotypic determination of HIV-2 coreceptor usage. Journal of the International AIDS Society. 2016;19(7).
  • Doering M, Borrego P, Büch J, Martins A, Friedrich G, Camacho RJ, et al. A genotypic method for determining HIV-2 coreceptor usage enables epidemiological studies and clinical decision support. Retrovirology. 2016;13(1).
  • Doerner T, Strand V, Cornes P, Goncalves J, Gulacsi L, Kay J, et al. The changing landscape of biosimilars in rheumatology. Annals of the Rheumatic Diseases. 2016 Jun;75(6):974–82.
  • Duarte A, Rodrigues JC, Reis L, Nogueira I, Carvalho P, Paulino S, et al. Risk assessment for public health from human interaction with ornamental waters. Microscopy and Microanalysis. 2016;22:12–3.
  • Falcon-Neyra L, Palladino C, Gomez MLN, Soler-Palacin P, Gonzalez-Tome MI, De Ory SJ, et al. Off-label use of rilpivirine in combination with emtricitabine and tenofovir in HIV-1-infected pediatric patients A multicenter study. Medicine (United States). 2016;95(24).
  • Fernandes S, Labarde A, Baptista C, Jakutytè L, Tavares P, São-José C. A non-invasive method for studying viral DNA delivery to bacteria reveals key requirements for phage SPP1 DNA entry in Bacillus subtilis cells. Virology. 2016;495:79–91.
  • Fernandes S, Sao-Jose C. More than a hole: the holin lethal function may be required to fully sensitize bacteria to the lytic action of canonical endolysins. Molecular Microbiology. 2016;102(1):92–106.
  • Figueiredo NL, Canário J, O’Driscoll NJ, Duarte A, Carvalho C. Aerobic Mercury-resistant bacteria alter Mercury speciation and retention in the Tagus Estuary (Portugal). Ecotoxicology and Environmental Safety. 2016;124:60–7.
  • Giria M, Santos L, Louro J, de Andrade HR. Reverse genetics vaccine seeds for influenza: Proof of concept in the source of PB1 as a determinant factor in virus growth and antigen yield. Virology. 2016;496:21–7.
  • Godinho-Santos A, Hance AJ, Goncalves J, Mammano F. CIB1 and CIB2 are HIV-1 helper factors involved in viral entry. Scientific Reports. 2016;6.
  • Goncalves J, Araujo F, Cutolo M, Fonseca JE. Biosimilar monoclonal antibodies: preclinical and clinical development aspects. Clinical and Experimental Rheumatology. 2016;34(4):698–705.
  • Goncalves J, Graca L, Moreira JN, Figueiredo I V, Scappaticci F, Eurico Fonseca J, et al. Development of e-learning platform about biologic drugs with focus on monoclonal antibodies. International Journal of Clinical Pharmacy. 2016;38(2):519–20.
  • Hurt AC, Besselaar TG, Daniels RS, Ermetal B, Fry A, Gubareva L, et al. Global update on the susceptibility of human influenza viruses to neuraminidase inhibitors, 2014–2015. Antiviral Research. 2016;132:178–85.
  • Justino GC, Pinheiro PF, Roseiro APS, Knittel ASO, Gonçalves J, Justino MC, et al. Camphor-based CCR5 blocker lead compounds-a computational and experimental approach. RSC Advances. 2016;6(61):56249–59.
  • Kushkevych I, Kollar P, Ferreira AL, Palma D, Duarte A, Lopes MM, et al. Antimicrobial effect of salicylamide derivatives against intestinal sulfate-reducing bacteria. Journal of Applied Biomedicine. 2016;14(2):125–30.
  • Louro J, Correia V, Rebelo-de-Andrade H. A cost-efficient solution: Reagent comparison guide for neuraminidase inhibition assay. Journal of Clinical Virology. 2016;82(S):S119–20.
  • Machado D, Pires D, Perdigão J, Couto I, Portugal I, Martins M, et al. Ion channel blockers as antimicrobial agents, efflux inhibitors, and enhancers of macrophage killing activity against drug resistant mycobacterium tuberculosis. PLoS ONE. 2016;11(2).
  • Martin F, Palladino C, Mateus R, Clemente S, Gomes P, Taveira N. Evaluation of an In-house Molecular HIV-1 Test to Assess Mother-to-Child HIV-1 Transmission in Angola (the APEHC Cohort). JAIDS-Journal of Acquired Immune Deficiency Syndromes. 2016 Jan;71(1):73.
  • Nascimento M, Rodrigues JC, Reis L, Nogueira I, Carvalho PA, Brandao J, et al. Pathogens in Ornamental Waters: A Pilot Study. International Journal of Environmental Research and Public Health. 2016;13(2).
  • Perdigão J, Maltez F, Machado D, Silva H, Pereira C, Silva C, et al. Beyond extensively drug-resistant tuberculosis in Lisbon, Portugal: a case of linezolid resistance acquisition presenting as an iliopsoas abscess. International Journal of Antimicrobial Agents. 2016;48(5):569–70.
  • Perdigão P, Gaj T, Santa-Marta M, Barbas CF, Goncalves J. Reactivation of latent HIV-1 expression by engineered TALE transcription factors. PLoS ONE. 2016;11(3).
  • Phelan JE, Coll F, Bergval I, Anthony RM, Warren R, Sampson SL, et al. Recombination in pe/ppe genes contributes to genetic variation in Mycobacterium tuberculosis lineages. BMC Genomics. 2016;17.
  • Piedade D, Azevedo-Pereira JM. MicroRNAs, HIV and HCV: a complex relation towards pathology. Reviews In Medical Virology. 2016;26(3):197–215.
  • Piedade D, Azevedo-Pereira JM. The role of microRNAs in the pathogenesis of herpesvirus infection. Viruses. 2016;8(6).
  • Pineda-Peña A-C, Varanda J, Sousa JD, Theys K, Bártolo I, Leitner T, et al. On the contribution of Angola to the initial spread of HIV-1. Infection, Genetics and Evolution. 2016;46:219–22.
  • Pires D, Marques J, Pombo JP, Carmo N, Bettencourt P, Neyrolles O, et al. Role of Cathepsins in Mycobacterium tuberculosis Survival in Human Macrophages. Scientific Reports. 2016;6.
  • Pires D, Zagalo A, Santos C, Cota de Medeiros F, Duarte A, Lito L, et al. Evolving epidemiology of carbapenemase-producing Enterobacteriaceae in Portugal: 2012 retrospective cohort at a tertiary hospital in Lisbon. Journal of Hospital Infection. 2016;92(1):82–5.
  • Santos-Costa Q, Azevedo-Pereira JM, Lopes MM, Rocheta M. A new combination of old techniques for HIV disease progression - qRT-PCR and ONEp-PCR. Journal of Clinical Virology. 2016;82(S):S43.
  • Sardari S, Portugal I, ALKafri A, Moradi D, Ghavami G. Fragment-based de novo design of antimycobacterial agents and in vitro potency evaluation. Combinatorial Chemistry and High Throughput Screening. 2016;19(3):238–45.
  • Solo-Gabriele HM, Harwood VJ, Kay D, Fujioka RS, Sadowsky MJ, Whitman RL, et al. Beach sand and the potential for infectious disease transmission: Observations and recommendations. Journal of the Marine Biological Association of the United Kingdom. 2016;96(1):101–20.
  • Vibe CB, Fenaroli F, Pires D, Wilson SR, Bogoeva V, Kalluru R, et al. Thioridazine in PLGA nanoparticles reduces toxicity and improves rifampicin therapy against mycobacterial infection in zebrafish. Nanotoxicology. 2016;10(6):680–8.

º lugar da 2.ª edição do Prémio Janssen Inovação

Lugar do Prémio Janssen Inovação 2016
Bolsa do Programa Gilead GÉNESE 2016
“Top Communication Award”
Best Oral Presentation. 8th Biennial Congress of the Spanish Society for Gene and Cell Therapy.
Best Oral Presentation. 8th iMed.ULisboa Postgraduate Students Meeting. 2016.


Edifício F e Edifício Carlos da Silveira
Tel.: +351 217 946 400
Extensão interna: 14512
E-mail: joao.goncalves@ff.ulisboa.pt