Master in Medicinal and Biopharmaceutical Chemistry

Analytical Tools for Small Molecules

ECTS

5

Learning outcomes

The main goals of this course are to train the students with the skills and knowledge on structural elucidation of organic compounds. Specifically, this
course is designed to provide students the learning of fundamentals of spectroscopic methods emphasizing nuclear magnetic resonance and mass
spectrometry and their application in the identification and structural characterization of organic compounds.

Syllabus

• Spectroscopic methods. Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR): the origin of the signal; magnetic and chemical equivalence; chemical shift; spin-spin splitting; coupling constants. Overhauser nuclear effect.
• Carbon Nuclear Magnetic Resonance Spectroscopy (13C-NMR). Bidimentional Nuclear Magnetic Resonance. Homonuclear 1H-1H (COSY e de NOESY) and heteronuclear 1H-13C (HMQC e HMBC) correlation spectra.
• Mass Spectrometry. Ionization methods. Interpretation of mass spectra obtained by electron impact. Mass spectrometry of biomolecules - applications. Hyphenated techniques for separation and analysis of mixtures.
• Ultraviolet–visible spectroscopy
• Infrared Spectroscopy.

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