Master in Laboratory Medicine

Complemmentary Immunology

ECTS

6

Learning outcomes

The CU has as an overall objective for the student to acquire knowledge necessary to the understanding of the cellular and molecular mechanisms involved in the immune response that leads to the disease by: lack of response (immunodeficiency), exaggeration or inadequacy (hypersensitivity), aberrant response (immunoproliferative disorders and neoplasia syndromes) and dysfunction (autoimmune disease). The CU as is integrated in a Master's Degree in Clinical Analysis includes a component dedicated to diagnostic applications. In this way, the CU is complete with a strong laboratory component on basic concepts and clinical applications of some advanced immunological techniques.
The CU will allow the acquisition of skills in:1) Identify, interpret and describe the cellular and molecular mechanisms responsible for changes in the immune response and its implications; 2) Interpret the changes in immune response detected in biological samples, in the light of the knowledge acquired in CU.

Syllabus

Theoretical Teaching:

  • Immune System and Immune Response
  • The Complement System
  • The Major Histocompatibility System
  • The Inflammatory Response
  • Immunobiology of tumors: (Bio)tumor markers: Clinical and laboratory
  • Autoimmunity
  • Hypersensitivity reactions: Classification of Gell and Coombs Kay
  • Immunodeficiencies

Practical Teaching:

  • Laboratory methods in Immunology: precipitation methods, RIA, EIA and variants (ELFA, Western Blot, etc), flow citometry
  • Identification of autoantibodies
  • Autoimmune diseases ­ practical Case: autoimmune Diabetes
  • Identification of paraproteinemias: Importance for the determination of light chains in the Diagnosis of Multiple Myelomas
  • Tumor Markers: practical Cases

Teaching Laboratory:

  • Determination of immunoglobulins: G, A and M
  • Determination of complement components by ID
  • Determination of biomarker by ELISA
  • Determination of IgE by ELFA
  • Typing HLA class I and II
  • Immunophenotyping of peripheral blood: lymphocyte populations: B, T and NK

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